anti-psmad1/5/9  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: The tectum transversum(TTR) maintains patency of the developing coronal suture

doi: 10.1101/2025.03.30.646197

Figure Lengend Snippet: (A) Sample overview shows regions of interest, including CS, Dura, Osteo and TTR. (B) Confirmed expression of spatially unique marker genes. (C) Pathway analysis of gene expression in CS of littermate control( WT ) vs Col2a1Cre;R26R DTA (MT). (D) Overall communication strength WT vs MT (thicker line means more communication, line color denotes where signal is coming from). A stronger interaction between Dura and CS was observed in the MT . (E) BMP signaling is significantly strengthened in MT , while none was predicted in littermate control. (F) The ligand receptor pairs driving Bmp signaling in WT and MT . (G) Average expression of BMP signaling components and downstream targets. (H) Alterated expression of Fstl1 and Inhba in WT and MT . (I, J) Immunostaining with anti-pSmad1/5/9 on transverse section of WT and MT at comparable level. Slides were counterstained with DAPI(blue). Blank arrows point to pSmad1/5/9 signal in WT TTR. Red arrows point to increased pSmad1/5/9 signal in CS of MT . (K-L) In situ hybridization with antisense probe of Spp1(K, L) and Msx1(M, N) on coronal sections of WT (K, M) and MT (L, N). Ectopic expression of Bmp signaling targets Spp1 and Msx1 in MT CS is marked by red arrows.

Article Snippet: The following primary antibodies were used in the present study: anti-Runx2 (Cell Signaling Technology 12556, 1:200) anti-Sp7 (Abcam ab94744, 1:200), anti-ColX (Thermo Fisher 14-9771-82, 1:200), anti-cleaved Caspase 3 (Cell Signaling Technology 9664, 1:400) and pSmad1/5/9 (Cell Signaling Technology 13820, 1:200).

Techniques: Expressing, Marker, Gene Expression, Control, Immunostaining, In Situ Hybridization